The group of Professor Teruyuki KOMATSU and others have prepared covalent core–shell structured protein clusters of hemoglobin (Hb) and human serum albumin (HSA) (HbX-HSAm) (m = 2, 3) with by linking Hb surface lysins to HSA cysteine-34 via an α-succinimidyl-ε-maleimide cross-linker. Structures were determined by TEM. This O2-carrying hemoprotein cluster with strongly negative surface net charge, high O2 affinity, and NADH-dependent reductase unit can support a new generation of molecular architecture for red blood cell substitutes.

Daiki TOMITA et al, Biomacromolecules, April 29, 2013, DOI: 10.1021/bm400204y