Using 3D ProSeedTM hydrogels for 3D culture of iPSC-derived CNS.4U neural cells and confocal high-content imaging, the company has developed a quantitative high-throughput assay which enables assessment of the viability and morphological changes in 3D neuronal cultures. Higher resolution and multi-parametric analysis allows neurite and single cell counting and statistically characterizes neurite development and branching in 3D. 2D and 3D analysis enable characterization of neuronal networks and provide quantitative measurements that can be used to define IC50 values and compare toxicities of various compounds.
Society for Laboratory Automation and Screening (SLAS) 2017 Washington meeting, 3D matrix poster presentation
3DMatrix offers iPSC neuronal cultures for toxicity assays