A research team led by Kao’s Life Science Research Institute has developed a highly efficient homologous recombination procedure for Rhizopus sp., based on TALEN genomic editing with short-length single-stranded DNA. Using this technology, a pyruvate decarboxylase (pdc) gene behind an adh promoter was inserted into the Rhizopus genome, leading to 1,2-fold increase of fumaric acid production from glucose.
Lectures at Annual Meeting of JSBBA in Nagoya, March 16,, 2018
Kao Co. improves fumaric acid production in Rhizopus sp. by genome editing