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In the red sea bream, 14 bases of the myostatin gene suppressing muscle hyperplasia were deleted, using CRISPR/Cas technology. The average amount of the edible portion was increased about 1.2 times, and the ratio of weight gain to ingested feed improved by 14%. Since the bone volume is reduced by about 31%, it is thought that the energy to make bones goes to the muscles. Pertinent research was done at Kinki University and Kyoto University, and commercialization is by Regional Fish https://regional.fish/en/, started by crowd-funding. The Ministry of Agriculture and Fisheries confirmed that the fish is not subject to the Cartagena Law, and the the Ministry of Health, Labor and Welfare confirmed that the fish conform to the Food Sanitation Law. Within a crowdfunding campaign, three types of red sea bream food are presently offered, with prices ranging from 10,000 yen to 15,000 yen for single items and 30,000 yen (77 – 230 €) for a three-item assortment. In addition to red sea bream, Regional Fish Co. plans to develop and market new varieties of marine products such as fish and crustaceans improved by genome editing.
This is the second report of genome-edited foods in Japan, following tomatoes with increased GABA content after deleting a decarboxylase gene, developed and marketed by Sanatech Seed, a startup from the University of Tsukuba.
Nikkei Biotech news release, September 21, 2021
Within its biotech-related 74 billion Yen (about 570 million €) budget for FY2022, the ministry aims to strengthen production capacity and improve sustainability of the domestic agriculture, forestry, and fisheries industries. Topics include:
- low-temperature storage systems for tea leaves and late-season tea varieties
- technologies for early egg collection, artificial seedling cultivation and aquaculture of eel and bluefin tuna,
- technology to reduce greenhouse gas emissions from livestock production. Development of technologies to reduce greenhouse gas emissions from livestock production, including breeding methods to produce low-methane cattle
- powerful red tide response technology for fish and shellfish farming including red tide-resistant cultured fish
- technology to cope with damage caused by invasive alien species, including technology using environmental DNA,
- technology for carbon sink measures in agriculture, forestry and fisheries, e. g., technology for production and utilization of wood lignin-derived plastics
- green biotechnology industry using silkworm technology
- projects to realize food and dietary habits to extend healthy life expectancy
- crop varieties using genome editing technology
Nikkei Biotech news release, September 7, 2021
Toshiba and others explore a system to store personal genetic data from projects of the Tohoku Medical Megabank Organization (ToMMo) using a combination of quantum cryptography communication technology and secret distribution technology. The consortium has successfully conducted a demonstration experiment to distribute large-scale human genome analysis data to multiple locations for safe backup storage.
Nikkei Biotech news release, August 27, 2021
Grillus is a startup from the University of Tokushima that produces edible crickets and sells cricket-derived as „circular food“. Basic technology consists of mass production of a highly uniform albino strain of the Dipterocarpus cricket, which has been maintained at the University of Tokushima for generations. The crickets are then dried and grinded providing a highly nutritious powder of about 70% protein and 20% fat. Flavor comes from the aroma produced during the drying process. Presently, the company offers 3 products:
- “cricket ramen” made from crickets at a ramen restaurant in Tokyo
- “cricket miso ramen” made from crickets and miso at a ramen restaurant in Tokushima Prefecture, and
- “cricket rice crackers” with a shrimp-like flavor produced by MUJI Co.
The company is also considering to develop genome-edited crickets with improved taste, reduced allergens, strains that can be bred at high density, strains with a regulated metabolic system for functional components, and strains with higher nutritional value, e.g., increased content of specific amino acids.
Nikkei Biotech news release, August 27, 2021
© Nikkei Biotech
A consortium of ARPChem, Fujifilm, TOTO, Mitsubishi Chemicals and the University of Tokyo, supported by NEDO, has installed and operated, since August 2019, a demonstrator for photocatalytic water splitting and succeeded in separating and recovering high-purity „solar hydrogen“ from the mixture of hydrogen and oxygen generated by water decomposition. The system consists of a photocatalytic panel reactor connected so that it has a light-receiving area of 100m2 and a gas separation module with a built-in separation membrane. The top of this reactor is made of transparent glass and a strontium titanate photocatalyst sheet. There is a small gap of 0.1 mm between the photocatalyst sheet and the glass through which water is supplied for the reaction. The strontium titanate photocatalyst uses sunlight UV for water splitting, with a quantum yield of almost 100%. It is manufactured by spraying photocatalyst onto the substrate. The gas separation module can maintain stable gas separation by properly controlling the gas flow rate regardless of sunlight conditions, and was operated outdoors for 65 days without losing its functionality. In addition, due to the proper design of the photocatalytic reaction system, there was no spontaneous ignition or explosion during the outdoor test. In a separate project, catalysts and process technologies are being developed to synthesize C2 to C4 olefins from hydrogen produced from this module and carbon dioxide emitted from power plants and factories.
1. NEDO news release, August 28, 2021
2. Nishiyama, H., Yamada, T., Nakabayashi, M. et al. Photocatalytic solar hydrogen production from water on a 100 m2-scale. Nature (2021). https://doi.org/10.1038/s41586-021-03907-3
Japan’s Science and Technology Agency JST and NEDO, project house of the Ministry of Economics, Trade and Industry, offer an annual award to startups, financially supported by companies. The 2021 prize was awarded to
- Heartseed Co. https://heartseed.jp
Heartseed, a spin-off of Kyoto University, develops cardiac regeneration medicine for severe heart failure, using iPS cells. The company has established a method for mass producing cardiomyocytes from allogenic tissue, supplied by the Center for iPS Cell Research and Application CiRA in Kyoto. A clinical trial for Phase 1/2 study obtained permission in March 2021, and will start later this year. On June 1, 2021 Heartsed announced that it has entered into an exclusive technology collaboration and license agreement with Novo Nordisk of Denmark for its allogenic cardiomyocytes. The reward is supported by Ajinomoto which has optimised the medium to culture the cardiomyocytes.
2. Regional Fish Co. https://regional.fish
The company, a spin-off of Kyoto University, aims to revolutionize Japan’s fisheries industry through the combination of accelerated fish breeding, based on genome editing, and smart, land-based aquaculture. For the latter aspect, it is supported by Ebara Co., a pump company.
3. OriCiro Co. https://www.oriciro.com
The company, supported by Rikkyo University, is focused on DNA synthesis and amplification technology based on amplifying long DNA strands without using cells. Its in vitro complete reconstitution system of genome replication cycles uses purified proteins and is claimed to be the only company in the world that can amplify genome-size long DNA strands as cyclic molecules, e.g., for the use in synthetic biology.
4. Raputya Robotics Co. https://www.rapyuta-robotics.com/ja/company/
The company, supported by ETH Zurich, develops cloud computing platforms for robots and is based on the vision that within 5 years, 10% of all robots globally will be connected to their control platform. Industrial support is from Monoful Co. which provides robotics solutions and develops a cloud robotics platform.
5. Matrixome Co. https://matrixome.co.jp/en/
The company, a spin-off of Osaka University, commercializes and sells laminin E8 fragment proteins, which are useful substrates for cell culture as surface-cultured pluripotent cells of clinical grade can be easily removed from this protein-engineered surface. Industrial support is from Nippi Co., which produces these surface proteins.
6. RTI-cast Co. https://www.rti-cast.co.jp
The company is a spin-off of Tohoku University and develops a Tsunami disaster information system that immediately predicts tsunami inundation damage at the time of an earthquake based on supercomputer terrain modelling and crustal movement observation. Industrial partners are Kokusai Kogyo, NEC, and EITO.
The program supported by METIs project house NEDO looks for an implementation in 2030 and beyond, but has organized another industry-academia consortia to improve fundamental technologies related to SOFC and PEFC fuel cells. It runs from FY2020 – FY2024 (planned), with a FY2021 budget of 6.67 billion yen (50 million €). The targets are
- 6 projects on separator technologies
- 7 projects on non-platinum electrocatalysis
- 4 projects on improved hydrogen storage
- new applications of fuel cells in agriculture, coastal vessels, drones etc.
A detailed list of projects and project partners is available.
NEDO news release, July 15, 2021
Heartseed has granted Novo the exclusive rights to develop, manufacture, and sell HS-001, regenerated cardiomyocytes derived from heterologous iPS cells in the world except Japan. In Japan, Heartseed will continue to develop the product independently, while retaining the rights to develop, manufacture and market it. After obtaining approval, Heartseed will market the product jointly with Novo’s Japanese subsidiary. License fees may add up to up to 65.5 billion yen (500 million €). In the second half of 2021, HeartSeed plans to start a domestic Phase 1/2 clinical trial (LAPiS trial) for severe heart failure caused by ischemic heart disease as a corporate trial.
Nikkei Biotech news release, July 14, 2021
The project titles and the responsible project coordinators are
1. Demonstrator for agrochemical active natural products produced by filamentous fungi with improved productivity Meiji Seika Pharma Co.
2. Demonstrator for implementation of infection control technology using super heat-resistant protease Saraya Co., Osaka University
3. Demonstrator for bio-isopropanol production using Corynebacteria RITE Green Earth Institute Co.
4. Demonstrator for the microbial production of glycyrrhetinic acid and its analogues Sumitomo Chemical Co., Osaka University
5. Demonstrator for production of an antibacterial cyclic lipopeptide by Bacillus species Kaneka Corporation
6. Development of an efficient bio-production method for imidazole dipeptides okai Bussan Co., Waseda University
7. Automated production system for health food additive 10-hydroxy-cis 12-octadecenoic acid HYA®50 Noster Co.
8. Demonstrator for a second generation sugar production system in a paper mill Mitsubishi Paper Mills, Inc.
9. Development of a new wastewater treatment process that enables biomethanation and biofuel production Taisei Corporation, Saitama University, Chubu University, Kazusa DNA Research Institute
10. Demonstrator for a highly integrated sugar production system using epigenetic metabolic conversion technology ACPLANTA Corporation, NTokyo Institute of Technology, Takasaki University of Health and Welfare
11. High-yield production technology of oxidized Glutathione by E. coli fermentation Kaneka Corporation
12. Demonstrator for high-efficiency production of human long-chain ceramide Fukuoka Soy Sauce Brewing Cooperative
13. Mass production system for Potato Cysticercus Hatchery Promoter (PCN-HF) HOKUSAN CO.
14. Development of fermentation technology for polyamide raw materials Toray Industries, Inc.
The projects will run for a maximum period of 5 years, with a budget (including self-financing) of 20 – 100 million JP¥ per year (100 million JP¥ is about 7.7 million €)
NEDO news release, July 7, 2021
With Japan Groundwater Development Co., and General Heat Pump Industry Co. as contractors, NEDO had built a demonstration facility as „ZEB” (zero-emission building) in Yamagata City, Yamagata Prefecture, to verify the ZEB adaptability of a total heat supply system that utilizes high-efficiency aquifer heat storage.
Number of floors 2 floors above ground
Building area 285 qm
Total floor area 562.5 qm
Air-conditioning load 64 W / qm
Heating load 35 W / qm
The building uses a 30.7 kW solar power generation facility and a solar water heater as energy creation, the wall thickness has been increased to 300mm to enhance the heat insulation effect. The ventilation system is a total heat exchange system, and the lighting is LED lighting. In addition, an external blind that blocks up to 82% of solar radiant heat is additionally installed on the southwest window. By making the vacuum tube type solar water heater an antifreeze circulation type, it is not easily affected by the outside air temperature, and it is expected that it will exhibit a certain heat collecting capacity even in winter when the sun comes out.
NEDO news release, July 6, 2021
2-7 University of Tokyo team edits chloroplast genome of a plant using procedures not subject to Cartagena law regulation
Shinichi Arimura and colleagues have used a TALEN tool for genome editing developed in Japan. The gene encoding a citidine deaminase (CD) is linked to the TALEN domain, and a gene encoding for delivery to the chloroplast is ligated. Using this method, single nucleotide substitutions at the target site of a chloroplast genome could be achieved in all chloroplasts with 1000 copies per cell. Furthermore, in subsequent generations, no segrigants containing foreign genes were observed. Genome modification of chloroplasts has been possible since around 1990 through introducing genes by a particle gun. However, molecular breeding that is not regulated by the Cartagena Law had not yet been realized.
Nikkei Biotech news release, July 2, 2021
Based on technology of the Forest Research Institute, the plant will be operated by a consortium consisting of seven companies, including Ligno Materia (Shinjuku, Tokyo, President Zenji Miura). A method was developed for extracting lignin using polyethylene glycol (PEG) and acid. As a result, “glycol lignin” in which PEG is bound to lignin is produced. Modified lignin refers to this glycol lignin. The advantage is that the binding of PEG makes it easier to process by heat and the stability after processing is also high. In addition, by using Japanese conifer trees (Sugi), which have a uniform lignin composition, it is believed that the problematic composition variation can be eliminated. According to experts, the annual consumption of plastics in Japan is about 10 million tons, while the annual production capacity of modified lignin calculated from the growth of sugi forests in Japan is more than 2 million tons.
Nikkei Biotech news release, July 1, 2021
In this project on CO2 capture, utilization, and storage (CCUS), research and development on liquefaction and storage systems for CO2 suitable for long-distance and mass transportation will be done. From around the end of fiscal 2023, the project will verify the technologies necessary for the operation and management of an integrated transport system in which CO2 emitted from a coal-fired power plant in Maizuru City, Kyoto Prefecture, on an annual scale of 10,000 tons will be liquefied at a shipping terminal, transported by ship, and received at a terminal in Tomakomai City, Hokkaido. This is expected to be the world’s first demonstration test of marine transportation of liquefied CO2 for the purpose of CCUS.
Project period: FY2021 – FY2026 (planned)
Budget: 16 billion yen (planned) (equivalent to 100 million €)
NEDO news release, June 22, 2021
Formic acid can be prepared through hydrogenation of CO2. The hydroxycarbonylation reaction of alkenes using formic acid has conventionally been catalyzed by a rhodium complex, Rh2Cl2(CO)4, and additives, triphenylphosphine (PPh3) and methyl iodide (CH3I), which have high environmental impact and toxicity, and p-toluenesulfonic acid hydrate (p-TsOH, H2O), which promotes the decomposition of formic acid. Under a contract from NEDO, the company has developed an improved catalyst, RhHI2(CO)(PPh3)2 in acetic acid, a safe and environmentally friendly for the synthesis of carboxylic acids in acetic acid as solvent. As a model reaction, ydroxycarbonylation of cyclohexene was studied.
NEDO news release, June 18, 2021
NEDO has commissioned Mitsubishi Power, JERA Corporation, Toyo Engineering, and the Japan Aerospace Exploration Agency (JAXA) for the development of fuel production technology using wood waste as raw material, and IHI Corporation for fuel production technology using microalgae as raw material, within a “Biojet Fuel Production Technology Development Project. The fuel is produced either by gasification of solid woody cellulose, or by hydrogenation refining technology of oil derived from microalgae, both of which have been confirmed to conform to ASTM D7566, the international standard for aviation fuel. Based on the results of the NEDO project, fuel completed with both technologies was supplied to regular flights departing from Tokyo International Airport (Haneda Airport).
 Fuel produced from wood waste
Date: June 17, 2021
Flight number: Japan Airlines Flight 515
Segment: Tokyo International Airport to New Chitose Airport
Aircraft: Airbus A350-900
Fuel used Approx. 8,700l
2] Fuel produced from microalgae
(1) Date: June 17, 2021
Flight number: Japan Airlines Flight 515
Segment: Tokyo International Airport to New Chitose Airport
Aircraft: Airbus A350-900
(2) Date: June 17, 2021
Flight name: All Nippon Airways Flight 031
Segment: Tokyo International Airport to Osaka International Airport (Itami Airport)
Aircraft: Boeing 787-8
NEDO news release, June 18, 2021
24-6 Tsukuba University Hospital launches “Genome Dock” service for genome analysis data of healthy people
The service comprises initially 76 genes related to 18 diseases such as tumors, cardiomyopathy, congenital hearing loss, and immune disorders. Among these genes are APC, BRCA1 / 2, RET, SMAD4, TP53 and other genes familiar in cancer medicine, as well as the KCNH2 gene for hereditary arrhythmia / congenital long QT syndrome and the TTR gene for familial amyloidosis. , CYP2C9 gene related to drug metabolism, etc.. Once the test is on track, it is planned to expand to 193 genes and chromosomal structural abnormalities.
Phase 1 is a practical study (feasibility study). Phase 2 starting in July will comprise about 100 people at a cost of 400,000 yen per person. Phase 3, which is scheduled to start in January 2022, will upgrade to the hospital business, and plans for about 1000 people. At this stage, the cost per subject is assumed to be 500,000 to 650,000 yen.
Nikkei Biotech news release, June 24, 2021
11-6 Team at Tokyo University of Agriculture and Technology develops DNA aptamer that enhances luminol reaction more than 300 times
The group around Kazunori IKEBUKURO has used the SELEX method to develop an aptamer which binds to myoglobin and changes the three-dimensional structure of the active site. As a result, the enzymatic activity of myoglobin and the substrate specificity for luminol are increased, so that the luminescence signal generated by the peroxidase reaction over 300 times higher than usual. The group plans to combine their system with an aptamer for a target virus or pathogen so that the three-dimensional structure is assembled in the presence of the target. This would allow virus or pathogen detection technology by light.
Nikkei Biotech, June 11, 2021
The database is available from the National Institute of Technology and Evaluation of Products (NITE) under its Data and Biological Resource Platform (DBRP) website https://www.nite.go.jp/nbrc/dbrp/taglist and currently (June 2021) contains the following projects:
Title Proteome analysis_Aspergillus oryzae : Proteome analysis of Aspergillus oryzae
[English] The proteome of Aspergillus oryzae RIB40 (= NBRC 100959) grown under liquid culture and membrane-transfer culture have been analyzed.
Title Proteome analysis_Brevibacillus brevis : Proteome analysis of Brevibacillus brevis
[English] The proteome of Brevibacillus brevis 47 (= NBRC 100599) grown under T2 medium and minimal medium have been analyzed.
Title Proteome analysis_Aeropyrum pernix : Proteome analysis of Aeropyrum pernix
[English] The proteome of the aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1 (= NBRC 100138) grown at 90 ˚C has been analyzed.
Title Proteome analysis of Methanococcus maripaludis : Proteome analysis of Methanococcus maripaludis
[English] The proteome of methanogenic archaea, Methanococcus maripaludis strain OS7 (= NBRC 103642) with the iron corrosive property and its corrosion-defective mutant OS7mut1 (= NBRC 105638) have been analyzed.
Title Proteome analysis of Escherichia coli : Proteome analysis of Escherichia coli
[English] The proteome of Escherichia coli K-12 W3110 grown under glucose minimal medium has been analyzed.
Title Proteome analysis of Rhodococcus opacus: Proteome analysis of Rhodococcus opacus
English] The proteome of Rhodococcus opacus B4 (= NBRC 108011) and its mutants have been analyzed.
Title Proteome analysis of Kocuria rhizophila : Proteome analysis of Kocuria rhizophila
[English] The proteome of Kocuria rhizophila DC2201 (= NBRC 103217), which is the bacteria tolerant to organic solvents, grown under several conditions The proteome of Kocuria rhizophila DC2201 (= NBRC 103217), which is the bacterial tolerant to organic solvents, grown under several conditions
Title Proteome analysis of Anaerolinea thermophila: Proteome analysis of Anaerolinea thermophila
[English] The proteome of Anaerolinea thermophila UNI-1 has been analyzed.
Data Title Project (Proteome Analysis_Arthrospira platensis (Spirulina platensis))
[English] The proteome of Arthrospira platensis (Spirulina platensis) NIES-39 have been analyzed.
Title Proteome analysis_Gemmatimonas aurantiaca: Proteome analysis of Gemmatimonas aurantiaca
English] The proteome of Gemmatimonas aurantiaca T-27 have been analyzed.
Title Proteome analysis of Sulfurisphaera tokodaii: Proteome analysis of Sulfurisphaera tokodaii
English] The proteome of Sulfurisphaera tokodaii strain 7 (Sulfolobus tokodaii strain 7) has been analyzed.
Title Proteome analysis of Caldilinea aerophila: Proteome analysis of Caldilinea aerophila
English] The proteome of Caldilinea aerophila STL-6-O1 (NBRC 104270T) has been analyzed.
Title Proteome analysis of Tetragenococcus halophilus : Proteome analysis of Tetragenococcus halophilus
[English] We have analyzed the proteome of Tetragenococcus halophilus (= NBRC 12172) cultured with added salt concentration of 0% and 10%.
Institution Name Strain Management Group, Planning and Management Department, Production Control Center, AminoScience Division, Ajinomoto Co.
Title Aquatic Imperfect Bacteria Screening Project
Abstract To create new medical and agricultural resources from aquatic imperfect bacteria known to have unique metabolites, a library of aquatic imperfect bacteria isolated as a screening source
Institution National Institute of Advanced Industrial Science and Technology (AIST)
Title Development of genome and transcriptome analysis technology suitable for information analysis
Abstract In order to realize reliable RNA-Seq analysis, the project aimed to expand the number of RNA reference materials for spike-ins and to apply them to industrial microorganisms, as well as to establish a method for obtaining gene expression information for RNA that is considered to be of low quality by general-purpose evaluation indices.
Institution Ajinomoto Co.
Title Development of a rapid search technology for DNA sequence factor combinations using the Combi-OGAB method and machine learning
Abstract By creating a microbial library using the Combi-OGAB method and analyzing the data using machine learning, we extracted the laws necessary for gene sequence design and high production of target substances. Specifically, by regulating the expression intensity of limonene biosynthetic enzymes in E. coli, we extracted the bottleneck enzymes and the rules for high production.
Institution Osaka University
Title Proteome analysis technology development
Abstract We constructed a multiple reaction monitoring (MRM) assay to measure the expression levels of E. coli central metabolic enzyme proteins. Specifically, we obtained proteome data by performing nano-LC-MS/MS analysis of tryptic digested samples of E. coli ASKA strain (one gene overexpression strain) and wild strain.
Institution Name Kobe University
Title Development of high-throughput microbial construction and evaluation technology
Abstract We developed a semi-automated transformation technology that can obtain several thousand transformants at once. Carotenoid pigment (lycopene or β-carotene) biosynthesis pathway plasmids were comprehensively introduced into gene-disrupted strains (approximately 4,000 strains of E. coli and 5,000 strains of budding yeast), and carotenoid productivity was comprehensively evaluated and data obtained by image analysis of bacterial coloration.
Institution Name Kobe University
Title Metabolome analysis technology development
Abstract Project Summary: In the course of developing analysis technology to obtain metabolome data that contributes to metabolic pathway design with high accuracy and high throughput, we established a metabolome measurement method using LC/MS/MS and developed an “automatic pretreatment system.
Institution Name University of Tsukuba, Nikon Instec Co.
Title Development of production technologies for high-functional products using plants and other organisms / Development of information analysis systems that contribute to the creation of highly productive microorganisms
Abstract We succeeded in predicting the amount of oil and fat produced by each cell by obtaining the single-cell autofluorescence profile of oil-producing yeast using the single-cell autofluorescence profile analysis platform.
Organization National Institute of Technology and Evaluation (NITE)
Title Development of design and optimization methods for novel metabolic pathways
Abstract Aiming to design new metabolic pathways for carotenoids and alkaloids using the metabolic pathway design platform tool we developed, we evaluated the carotenoid production culture of NBRC strains, obtained enzyme gene information that is expected to improve the productivity of these substances from genome information, literature and patent information, and verified the introduction effect. In addition, we obtained information on enzyme genes that are expected to improve the production of these substances from genome information, literature, and patent information, and verified the effects of their introduction.
Institution Asahi Kasei Pharma Co.
Title Efficacy verification by improving the productivity of cholesterol esterase
Abstract Cholesterol esterase is used for the diagnosis of blood cholesterol. In order to improve the productivity of cholesterol esterase producing strain Burkholderia stabilis, we conducted genome analysis of the producing bacteria. In addition, DNAseq and RNAseq analyses were conducted to search for genes that contribute to productivity.
Institution Nagaoka University of Technology
Title Validation of simultaneous control of production of useful proteins using filamentous fungi
Abstract T. reesei produces several extracellular enzymes, and omics analysis of the standard strain and the industrial production base strain was conducted in order to vary the production of each enzyme. Gene expression network analysis using the omics data was used to find the factors that change the enzyme components and to verify the disruptive effects.
Project URL: https://www.jba.or.jp/nedo_smartcell/proof/01.php
Institution Kobe Natural Products Chemistry Ltd. and National Institute of Advanced Industrial Science and Technology
Title Verification of the effectiveness of enzyme design technology using monoterpenoid oxidase such as limonene ~Development of enzyme modification technology using MD simulation~.
Abstract Regioselectivity and productivity are sometimes issues when using enzymatic reactions. Therefore, in collaboration with the National Institute of Advanced Industrial Science and Technology (AIST), we have developed a method for predicting enzyme modification sites that improve reaction regioselectivity using molecular dynamics (MD) simulation. In addition, the oxidation of monoterpenoids such as limonene was used as a model case, and the function of the modified enzyme was evaluated using P450 in the Kobe Natural Products Chemistry cytochrome P450 library.
Institution Name Mitsubishi Chemical Corporation
Title Validation of Metabolic Analysis Technology by Improving the Productivity of Useful Isoprenoids
Abstract In order to breed microbial strains that produce high levels of isoprenoid compounds, we analyzed the products of various breeding strains and their expression levels under a number of culture conditions, and used the results to extract candidate genes and reactions that contribute to solving bottlenecks.
Institution Name Research Institute of Innovative Technology for the Earth
Title Validation of Metabolic Analysis Technology by Improving the Productivity of Useful Aromatic Compounds Using Bacillus subtilis
Abstract A smart cell design system was verified through the improvement of catechol productivity. In the process, we conducted an omics analysis of catechol-producing strains and shikimic acid-producing strains under various conditions, contributing to the improvement of the accuracy of metabolic pathway design technology, expression control network construction technology, and transporter search technology.
Project URL: https://www.jba.or.jp/nedo_smartcell/proof/02.php
Institution National Institute of Advanced Industrial Science and Technology
Title Efficacy Verification by Controlling Pigment Production Using Red Yeast Rice Mold
Abstract To improve the productivity of red yeast rice pigment, a natural food pigment produced by red yeast rice mold, we conducted comprehensive gene expression analysis of the reference strain, mutant strains, and validation strains under various culture conditions to verify the effectiveness of genetic modification using a new mathematical model based on expression control network model. In addition, we clarified the pigment synthesis gene cluster and citrinin synthesis gene cluster by draft genome analysis of the reference strain, and constructed a biosynthetic model.
Institution National Institute of Advanced Industrial Science and Technology
Title Validation of a new production method for paprika-derived carotenoids using microorganisms
Abstract Targeting two industrially useful carotenoids (β-cryptoxanthin and lutein) derived from paprika, we selected enzymes involved in the efficient biosynthesis of these carotenoids and verified their production in E. coli and yeast. As a result, we succeeded in producing these two carotenoids in E. coli and yeast.
Project URL: https://www.jba.or.jp/nedo_smartcell/proof/07.php
Institution Niigata University of Pharmacy and Applied Life Sciences, Nagaoka University of Technology
Title Verification of the efficacy of oils and fats containing ω-3 polyunsaturated fatty acids by improving their productivity
Abstract We attempted to construct eicosapentaenoic acid C20:5 (EPA)-producing strains using two kinds of oil and fat yeasts (Lipomyces starkeyi and Rhodosporidium toruloides) and created EPA-producing L. starkeyi. In addition, we identified genes that improve oil and fat production by constructing an expression regulatory network model using transcriptome analysis data of oil and fat accumulation mutants, metabolome analysis, proteome analysis, and genome comparison analysis, and created a high-producing strain by utilizing these genes.
Abstracts of related presentations at the Japan Society for Bioscience and Biotechnology
Development of high oil and fat producing strains of the oil and fat yeast Lipomyces starkeyi (https://ci.nii.ac.jp/search?q=9000291273015)
Expression analysis of genes related to fat and oil synthesis and degradation in a low-fat accumulation mutant strain of Lipomyces starkeyi (https://ci.nii.ac.jp/search?q=9000316621316)
Acquisition of fat and oil hyperaccumulating mutant strains of Lipomyces starkeyi using density gradient centrifugation (https://ci.nii.ac.jp/search?q=9000316621322)
Acquisition of fat and oil hyperaccumulating mutant strains of the industrial oil and fat yeast Lipomyces starkeyi (https://ci.nii.ac.jp/search?q=9000321425083)
Expression analysis of genes involved in fat and oil accumulation in the oil and fat yeast Rhodosporidium toruloides (https://ci.nii.ac.jp/search?q=9000316621333)
Improvement of oleaginous yeasts using informatics for the production of functional fats and oils (https://jsbba.bioweb.ne.jp/cgi-bin/jsbba_db/jsbba_summary.cgi?id=58970)
Identification of genes responsible for low fat accumulation in the oleaginous yeast Lipomyces starkeyi by comparative genomic analysis (https://jsbba.bioweb.ne.jp/cgi-bin/jsbba_db/jsbba_summary.cgi?id=57534)
Identification and functional analysis of novel regulators of lipid accumulation in the oleaginous yeast Lipomyces starkeyi by comparative genomic analysis (https://jsbba.bioweb.ne.jp/cgi-bin/jsbba_db/jsbba_summary.cgi?id=57532)
Identification and analysis of the causative gene of lipid hypoaccumulation mutants in the oleaginous yeast Lipomyces starkeyi (https://jsbba.bioweb.ne.jp/cgi-bin/jsbba_db/jsbba_summary.cgi?id=56884)
Project URL: https://www.jba.or.jp/nedo_smartcell/proof/03.php
Institution Name Ishikawa Prefectural University, Kobe University
Title Validation of a new production method for alkaloids and other compounds using microorganisms
Abstract Five new biosynthetic pathways from tyrosine to 3,4-DHPAA were established by information analysis. In addition, we searched for enzymes with similar functions to tyrosine hydroxylase and monoamine oxidase, which are the rate-limiting reactions in the reticulin biosynthetic pathway, and introduced plasmids. In addition, we constructed a genome-inserted reticulin-producing E. coli strain for practical use, and also produced a genome-inserted tyrosine and dopamine high-producing E. coli strain.
Project URL: https://www.jba.or.jp/nedo_smartcell/proof/04.php
Under an initiative of Japan’s Agency for Medical Research and Development AMED, this collaboration among six institutions and five sites will sequence and mutually utilize genomic cohort data of 366,000 people. The project is expected to promote the elucidation of genetic predisposition affecting the onset of diseases in the Japanese population and the construction of risk prediction models. It will also contribute to next-generation medical care, such as personalized medicine, personalized prevention, and risk prediction tailored to each individual’s constitution.
Outside of Japan, genomic cohort studies involving several hundred thousand to one million people are The Million Veteran Program (MVP), which started in 2021 in the U.S. with more than 820,000 participants, and the UK Biobank, which started in 2006 in the U.K. and targets British more than 500,000 volunteers.
The DNA microarrays used at the six institutions and five sites are the Geponica array developed by ToMMo for the TMM project and the TMC Illuminas array.
Nikkei Biotech news release, May 28, 2021