https://sj.jst.go.jp/news/202602/n0203-01k.html

https://www.sciencedirect.com/science/article/abs/pii/S1369703X25004152?via%3Dihub

 ≈, advancing personalized medicine

2026.02.03

A research group at Kobe University has developed a preservation solution which enables the undisrupted cryopreservation of human iPS cells cultured in sheet form. The newly developed cryopreservation solution is composed of dimethyl sulfoxide (a classic cryoprotectant), cell culture medium, water, D-proline, a synthetic polymer and sodium chloride.

D-proline enhances the freezing tolerance of iPS cells, and the synthetic polymer functions to prevent large ice crystals from growing inside cells. Prior to being frozen, the cells are briefly treated with an enzyme that weakens cell adhesion.

The method improves cryopreservation and thawing of iPS cells and can be automated using robots. The cells can be used immediately after thawing for research or treatment.