https://bio.nikkeibp.co.jp/atcl/news/p1/22/08/15/09819/
https://link.springer.com/article/10.1007/s44211-022-00161-z
The system developed by a joint research group from the RIKEN Center for Emergent Matter Science and Chiba University Graduate School of Medicine uses a synthetic polymer that is photoreactive and inhibits nonspecific adsorption which is coated on a chip, and viral proteins are placed on the chip in the form of spots. The proteins are then photo-cross-linked by ultraviolet irradiation, and fixed to the chip to create a microarray. The proteins used were the nucleocapsid protein of SARS-CoV-2 and a portion of the spike protein (receptor binding region) that differs for each mutant strain. When antibodies binding to each virus protein are present in the test serum (5 µL of blood collected from the fingertip), the microarray emits light , and by photographing the emitted image with a camera, the amount of antibody is measured by the intensity of the emission. The entire process of dropping blood onto the chip, reacting with reagents, washing, and detection is fully automated and takes only 8 minutes. The data obtained agreed with ELISA test results with a correlation of more than 90%.