Kaneka develops suspension culture method for iPS cells that suppresses hyperaggregation by adding phospholipids

The method, codeveloped with Yasuyuki SAKAI of the University of Tokyo, allows to cultivate up to 109 of human iPS cells per ml by a suspension culture to which lysophosphatidic acid or sphingosine monophosphate is added. When analyzed by FACS, SOD 2 and OCT 4 of the undifferentiated markers were positive in 90% or more of the cells. In addition, when differentiated from the aggregates into endoderm, SOX17 endoderm marker was positive in 80% or more of the cells.

Kaneka news release, March 14, 2017

Kaneka develops suspension culture method for iPS cells that suppresses hyperaggregation by adding phospholipids
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