The method, codeveloped with Yasuyuki SAKAI of the University of Tokyo, allows to cultivate up to 109 of human iPS cells per ml by a suspension culture to which lysophosphatidic acid or sphingosine monophosphate is added. When analyzed by FACS, SOD 2 and OCT 4 of the undifferentiated markers were positive in 90% or more of the cells. In addition, when differentiated from the aggregates into endoderm, SOX17 endoderm marker was positive in 80% or more of the cells.
Kaneka news release, March 14, 2017
Kaneka develops suspension culture method for iPS cells that suppresses hyperaggregation by adding phospholipids