In this study financed by AMED the trick was to substitute glutamate and glucose in the iPS culture medium. In such case, the ratio of residual undifferentiated iPS cells in the expanded cardiomyocytes intended for transplantation was less than 0.001 %. Clinical studies based on this finding were already announced.
China Bio news release, June 15, 2016
Keio University group finds culture conditions to produce highly pure iPS-derived human cardiomyocytes