When inhibitors for Wnt-signaling such as IWP-2 were added to the growth medium, the epi stem cells could be stably maintained in a “primed” pluripotent state, and directly and in good yields differentiated from the primed state.
Michihiko SUGIMOTO et al., Stem Cell Report available online 26 March 2015
Group at RIKEN Bioresource Center finds procedure to stabilize mouse epiblast stem cells